Recommendation for the Measurement of “Alanine Aminopeptidase” in Urine
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چکیده
منابع مشابه
Recommendation for the measurement of "alanine aminopeptidase" in urine.
A method is recommended for the measurement of the catalytic concentration of alanine aminopeptidase in the urine of man, rat, and dog, using L-alanine-4-nitroanilide as substrate. In currently used methods, substrate concentrations between 1.7 and 2.0 mmol/l are used. Kinetic experiments show, however, that the reaction is inhibited by substrate concentrations exceeding 0.8 (man), 0.3 (rat) an...
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15 صفحه اولElectrophoretic variants of alanine aminopeptidase, alkaline phosphatase, and gamma-glutamyltransferase in urine.
The three brush-border enzymes--alanine aminopeptidase (EC 3.4.11.2), alkaline phosphatase (EC 3.1.3.1), and gamma-glutamyltransferase (EC 2.3.2.2)--are present in the urine of healthy persons in two variants, a particulate form and a soluble one. They can be separated by electrophoresis in agarose gel and by ultracentrifugation. The particulate forms exhibit similar electrophoretic mobility, b...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1988
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1988.26.10.635